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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers
Autores:  Paim,Fabilene Gomes
Brandão,José Henrique Souza Galdino
Sampaio,Iracilda
Affonso,Paulo Roberto Antunes de Mello
Diniz,Débora
Data:  2014-12-01
Ano:  2014
Palavras-chave:  COI
Karyotype
South Atlantic fish
Ribossomal genes
Robertsonian rearrangements
Resumo:  Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of heterochromatin and GC-rich single nucleolar organizer regions (NORs) not syntenic to 5S rDNA clusters. These are the first data about DNA barcoding in parrotfish from the Brazilian province and the first refined chromosomal analysis in Scarinae, providing useful data to a reliable genetic identification of S. radians.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500007
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572014005000024
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.37 n.4 2014
Direitos:  info:eu-repo/semantics/openAccess
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